No evidence for a pathogen associated with pulmonary MALT lymphoma: a metagenomics investigation

نویسندگان

چکیده

Abstract Mucosa-associated lymphoid tissue (MALT) lymphoma is generally associated with chronic antigen stimulation: auto-antigens or of microbial origin. Only one study suggested association between Achromobacter xylosoxidans and pulmonary MALT lymphoma. We aimed to investigate the presence virus any infectious agents in by using metagenomic next-generation sequencing (mNGS). All lung samples were centrally reviewed. The t(11;18) (q21;q21) was evaluated FISH analysis. snap frozen large biopsies analyzed mNGS. After homogenization total nucleic acids (RNA DNA) extracted, amplified classified according their taxonomic assignment, after exclusion host DNA. included 13 from (mean age: 60.3 years, 7 women, 3 auto-immune background) 10 controls. diagnosis confirmed for samples, showed API2-MALT1 translocation (23%). No evidence a specific pathogen clearly identified group patients A. sequence 4/13 4/10 This did not find DNA RNA virus, fungi, parasite bacteria either stroma tumor cells.

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ژورنال

عنوان ژورنال: Infectious Agents and Cancer

سال: 2021

ISSN: ['1750-9378']

DOI: https://doi.org/10.1186/s13027-021-00351-w